RORB Recombinant monoclonal monoclonal antibody, PBS Only

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  WB analysis using 85404-4-RR (same clone as 85404-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85404-4-RR (RORB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85404-4-PBS in a different storage buffer formulation.

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  IP experiment of mouse eye using 85404-4-RR (same clone as 85404-4-PBS)

  IP result of anti-RORB (IP:85404-4-RR, 4ug; Detection:85404-4-RR 1:10000) with mouse eye tissue lysate 2680 ug. This data was developed using the same antibody clone with 85404-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85404-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85404-4-RR against Human RORB were performed. The affinity constant is 0.412 nM.

RORB Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 85404-4-PBS

Clone No.242832D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

NR1F2, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Retinoid-related orphan receptor-beta, RZRB

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Product Information

85404-4-PBS targets RORB in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11910

  Product name: Recombinant human RORB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 106-459 aa of BC051830

  Sequence: EQRQQQSGEAEALARVYSSSISNGLSNLNNETSGTYANGHVIDLPKSEGYYNVDSGQPSPDQSGLDMTGIKQIKQEPIYDLTSVPNLFTYSSFNNGQLAPGITMTEIDRIAQNIIKSHLETCQYTMEELHQLAWQTHTYEEIKAYQSKSREALWQQCAIQITHAIQYVVEFAKRITGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGSDDLVNEAFDFAKNLCSLQLTEEEIALFSSAVLISPDRAWLIEPRKVQKLQEKIYFALQHVIQKNHLDDETLAKLIAKIPTITAVCNLHGEKLQVFKQSHPEIVNTLFPPLYKELFNPDCATGCK

• Full Name: RAR-related orphan receptor B
• Calculated Molecular Weight: 459 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC051830
• Gene Symbol: RORB
• Gene ID (NCBI): 6096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92753
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400).