RORB Recombinant antibody

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  WB analysis using 85404-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85404-4-RR (RORB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85404-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85404-4-RR against Human RORB were performed. The affinity constant is 0.412 nM.

RORB Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85404-4-RR

Clone No.242832D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NR1F2, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Retinoid-related orphan receptor-beta, RZRB

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Tested Applications

• Positive WB detected in: mouse retina tissue, rat retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85404-4-RR targets RORB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RORB fusion protein Ag11910
• Full Name: RAR-related orphan receptor B
• Calculated Molecular Weight: 459 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC051830
• Gene Symbol: RORB
• Gene ID (NCBI): 6096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400).