POLR1A Recombinant antibody

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  WB analysis using 85633-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85633-1-RR (POLR1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85633-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85633-1-RR against Human POLR1A were performed. The affinity constant is below 1 pM.

POLR1A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85633-1-RR

Clone No.243082E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, DNA-directed RNA polymerase I subunit RPA1, EC:2.7.7.6

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, LNCaP cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85633-1-RR targets POLR1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: POLR1A fusion protein Ag14543
• Full Name: polymerase (RNA) I polypeptide A, 194kDa
• Calculated Molecular Weight: 1720 aa, 195 kDa
• Observed Molecular Weight: 190-195 kDa
• GenBank Accession Number: BC017115
• Gene Symbol: POLR1A
• Gene ID (NCBI): 25885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95602
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.