RPGR Polyclonal antibody

RPGR Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16891-1-AP

Synonyms

COD1, CORDX1, CRD, orf15, PCDX, RP15, RP3, RPGR, XLRP3



Tested Applications

Positive WB detected inmouse eye tissue, mouse liver tissue, SH-SY5Y cells, Y79 cells
Positive IHC detected inmouse eye tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16891-1-AP targets RPGR in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPGR fusion protein Ag10405
Full Name retinitis pigmentosa GTPase regulator
Calculated molecular weight 113 kDa
Observed molecular weight 100-105 kDa, 70 kDa
GenBank accession numberBC031624
Gene symbol RPGR
Gene ID (NCBI) 6103
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.

Protocols

Product Specific Protocols
WB protocol for RPGR antibody 16891-1-APDownload protocol
IHC protocol for RPGR antibody 16891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols