Tested Applications
| Positive WB detected in | mouse spleen tissue, HEK-293 cells, MCF-7 cells, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13010-1-AP targets RPIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3699 Product name: Recombinant human RPIA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-311 aa of BC015529 Sequence: MQRPGPFSTLYGRVLAPLPGRAGGAASGGGGNSWDLPGSHVRLPGRAQSGTRGGAGNTSTSCGDSNSICPAPSTMSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLILQYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDQWHKGIPIEVIPMAYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDWKFDRVHKWSEVNTAIKMIPGVVDTGLFINMAERVYFGMQDGSVNMREKPFC Predict reactive species |
| Full Name | ribose 5-phosphate isomerase A |
| Calculated Molecular Weight | 311 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC015529 |
| Gene Symbol | RPIA |
| Gene ID (NCBI) | 22934 |
| RRID | AB_10638301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49247 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPIA(Ribose-5-phosphate isomerase) is also named as RPI and belongs to the ribose 5-phosphate isomerase family. It is involved in pentose-phosphate shunt, non-oxidative branch. It is an enzyme in the pentose-phosphate pathway, appears to be determined by a single structural locus and is probably a dimer(PMID:7396409).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPIA antibody 13010-1-AP | Download protocol |
| WB protocol for RPIA antibody 13010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPIA antibody (Cat# 13010-1-AP) has 2 published citations. These appear in Int J Biol Sci (IF 11.7) and Cell Death Discov (IF 10.4). The research fields span colorectal cancer (DNA damage and growth inhibition via POU2F1 ubiquitination) and hepatocellular carcinoma (glycolysis reprogramming through the MDMX/14-3-3γ/FOXO1 axis). Both studies applied the antibody for WB and IHC in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitination | ||
Cell Death Discov MDMX reprograms glycolysis of hepatocellular carcinoma via 14-3-3γ/FOXO1. |
