Multi-rAb® RPL10A Multi-Recombinant antibody

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  WB analysis using RMX00046

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00046 (RPL10A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using RMX00046

  IP result of anti-RPL10A (IP:RMX00046, 4ug; Detection:RMX00046 1:10000) with HepG2 cells lysate 1600 ug.

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  IHC staining of mouse liver using RMX00046

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00046 (RPL10A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using RMX00046

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00046 (RPL10A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using RMX00046

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL10A antibody (RMX00046) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPL10A Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. RMX00046

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

60S ribosomal protein L10a, Csa 19, CSA-19, Large ribosomal subunit protein uL1, NEDD 6

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00046 targets RPL10A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: ribosomal protein L10a
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC006791
• Gene Symbol: RPL10A
• Gene ID (NCBI): 4736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P62906
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL10A (Ribosomal Protein L10a) is a component of the 60S subunit of the ribosome and belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. RPL10A is ubiquitously expressed in various tissues, it plays a crucial role in ribosome biogenesis and protein synthesis. In addition to its role in protein synthesis, RPL10A has been implicated in cell proliferation, embryogenesis, and organogenesis. PMID: 29953919