Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, SGC-7091 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86205-1-RR targets RPL13 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RPL13 fusion protein Ag1814 Predict reactive species |
Full Name | ribosomal protein L13 |
Calculated Molecular Weight | 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC014167 |
Gene Symbol | RPL13 |
Gene ID (NCBI) | 6137 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P26373 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL13 may be a plausible candidate tumor suppressor gene, as knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle[PMID:16786168].
Protocols
Product Specific Protocols | |
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WB protocol for RPL13 antibody 86205-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |