RPL13A Polyclonal antibody

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  WB analysis using 14633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14633-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14633-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 14633-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14633-1-AP (RPL13A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RPL13A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14633-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ribosomal protein L13a, Large ribosomal subunit protein uL13

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, HEK-293 cells, HeLa cells, RAW264.7 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14633-1-AP targets RPL13A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6212

  Product name: Recombinant human RPL13A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-203 aa of BC065236

  Sequence: MAEVQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGPYHFRAPSRIFWRTVRGMLPHKTKRGQAALDRLKVFDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGRLAHEVGWKYQAVTATLEEKRKEKAKIHYRKKKQLMRLRKQAEKNVEKKIDKYTEVLKTHGLLV

• Full Name: ribosomal protein L13a
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC065236
• Gene Symbol: RPL13A
• Gene ID (NCBI): 23521
• RRID: AB_10638610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P40429
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein L13a(RPL13a) is a member of the L13 ribosomal protein family and a structural component of the large 60S ribosomal subunit. It's a reference gene(RG) that stably expressed not only during differentiation, but also during expansion to higher passages. And it's suggested as a RG for a variety research field. RPL13a is a key component of the IFN-gamma-activated inhibitor of translation(GAIT) complex, which inhibits translation of a select group of inflammation-related transcripts via binding to rhe 3' UTR of target mRNA.once phosphorylated of Ser77 by ZIPK, RPL13a released from the complex.