Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14991-1-AP targets RPL14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6976 Product name: Recombinant human RPL14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC000606 Sequence: MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKASGKKA Predict reactive species |
| Full Name | ribosomal protein L14 |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC000606 |
| Gene Symbol | RPL14 |
| Gene ID (NCBI) | 9045 |
| RRID | AB_2285319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50914 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL14 antibody 14991-1-AP | Download protocol |
| IHC protocol for RPL14 antibody 14991-1-AP | Download protocol |
| IP protocol for RPL14 antibody 14991-1-AP | Download protocol |
| WB protocol for RPL14 antibody 14991-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPL14 Polyclonal antibody (Cat# 14991-1-AP) has 10 citations across journals including Cell Research, Cell Discovery, Nucleic Acids Research, Science Advances, EBioMedicine, Cell Reports, Biochemical Pharmacology, Journal of Biological Chemistry, and American Journal of Cancer Research. Research fields span DNA damage repair, ribosomal RNA biology, homologous recombination, CRISPR-Cas13 off-target effects, mitochondrial function, metabolic reprogramming, viral translation, neuropharmacology, and cancer progression.
| Species | Application | Title |
|---|---|---|
Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. | ||
Cell Discov The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination | ||
Nucleic Acids Res Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cells. | ||
Sci Adv CLUH maintains functional mitochondria and translation in motoneuronal axons and prevents peripheral neuropathy | ||
EBioMedicine Skeletal muscle reprogramming of metabolic, ribosomal and developmental pathways contributes to Roux-en-Y gastric bypass-induced adaptation in women. |
