RPL14 Polyclonal antibody

RPL14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14991-1-AP

Synonyms

60S ribosomal protein L14, CAG ISL 7, CTG B33, hRL14, L14, ribosomal protein L14, RL14, RPL14



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, rat liver tissue, HeLa cells, MCF-7 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14991-1-AP targets RPL14 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rice
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL14 fusion protein Ag6976
Full Name ribosomal protein L14
Calculated molecular weight 23 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC000606
Gene symbol RPL14
Gene ID (NCBI) 9045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL14 antibody 14991-1-APDownload protocol
IHC protocol for RPL14 antibody 14991-1-APDownload protocol
IF protocol for RPL14 antibody 14991-1-APDownload protocol
IP protocol for RPL14 antibody 14991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
riceWB

J Exp Bot

Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.

Authors - Yanyun Shi