RPL18 Polyclonal antibody

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  WB analysis of HeLa using 17029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17029-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17029-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17029-1-AP (RPL18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RPL18 Polyclonal Antibody for WB, ELISA

Cat No. 17029-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

60S ribosomal protein L18, ribosomal protein L18, RPL18

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

17029-1-AP targets RPL18 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10474

  Product name: Recombinant human RPL18 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-186 aa of BC009708

  Sequence: MGVDIRHNKDRKVRRKEPKSQDIYLRLLVKLYRFLARRTNSTFNQVVLKRLFMSRTNRPPLSLSRMIRKMKLPGRENKTAVVVGTITDDVRVQEVPKLKVCALRVTSRARSRILRAGGKILTFDQLALDSPKGCGTVLLSGPRKGREVYRHFGKAPGTPHSHTKPYVRSKGRKFERARGRRASRGY

• Full Name: ribosomal protein L18
• Calculated Molecular Weight: 188 aa, 22 kDa
• Observed Molecular Weight: 22-26 kDa
• GenBank Accession Number: BC009708
• Gene Symbol: RPL18
• Gene ID (NCBI): 6141
• RRID: AB_2878340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q07020
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	Virus Res; Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication; Authors - Qinghe Zhu ; KD Validated
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk
mouse	WB	Biomaterials; P-Pev: micelle-like complexes transformed from tumor extracellular vesicles by PEG-PE for personalized therapeutic tumor vaccine; Authors - Hongjian Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katie (Verified Customer) (05-23-2022)

Gives appropriate sized band in western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells - mouse