RPL18A Polyclonal antibody
RPL18A Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14653-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, human brain tissue, mouse lung tissue, PC-3 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
14653-1-AP targets RPL18A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RPL18A fusion protein Ag6251 |
Full Name | ribosomal protein L18a |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC066319 |
Gene symbol | RPL18A |
Gene ID (NCBI) | 6142 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RPL18A antibody 14653-1-AP | Download protocol |
IHC protocol for RPL18A antibody 14653-1-AP | Download protocol |
IF protocol for RPL18A antibody 14653-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Genetics Quantitative changes in gimap3 and gimap5 expression modify mitochondrial DNA segregation in mice. | ||
Sci Rep Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway. |