RPL18A Polyclonal antibody

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  WB analysis of HepG2 using 14653-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14653-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14653-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14653-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14653-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14653-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 14653-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 14653-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14653-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

RPL18A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14653-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human brain tissue, mouse lung tissue, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

14653-1-AP targets RPL18A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6251

  Product name: Recombinant human RPL18A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-176 aa of BC066319

  Sequence: MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF

• Full Name: ribosomal protein L18a
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC066319
• Gene Symbol: RPL18A
• Gene ID (NCBI): 6142
• RRID: AB_2181576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.; Authors - Haiping Tang
mouse	WB	Genetics; Quantitative changes in gimap3 and gimap5 expression modify mitochondrial DNA segregation in mice.; Authors - Riikka Jokinen
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
rat	WB	EMBO Rep; Molecular view of ER membrane remodeling by the Sec61/TRAP translocon; Authors - Sudeep Karki