RPL18A Polyclonal antibody

RPL18A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15751-1-AP

Synonyms

60S ribosomal protein L18a, ribosomal protein L18a, RPL18A

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Validation Data Gallery

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  WB analysis using 15751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15751-1-AP (RPL18A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15751-1-AP (RPL18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15751-1-AP

  IP result of anti-RPL18A (IP:15751-1-AP, 4ug; Detection:15751-1-AP 1:500) with Jurkat cells lysate 3200 ug.

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  IHC staining of human brain using 15751-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15751-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15751-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15751-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15751-1-AP targets RPL18A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL18A fusion protein Ag8251
• Full Name: ribosomal protein L18a
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC007512
• Gene Symbol: RPL18A
• Gene ID (NCBI): 6142
• RRID: AB_2181574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.