RPL18A Recombinant monoclonal antibody

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  WB analysis using 83928-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83928-1-RR (RPL18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 83928-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83928-1-RR (RPL18A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  Affinity and Kinetic Characterization of 83928-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83928-1-RR against Human RPL18A were performed. The affinity constant is below 1 pM.

RPL18A Recombinant Antibody for WB, IHC, ELISA

Cat No. 83928-1-RR

Clone No.240841B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, 240841B1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83928-1-RR targets RPL18A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6251

  Product name: Recombinant human RPL18A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-176 aa of BC066319

  Sequence: MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF

• Full Name: ribosomal protein L18a
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC066319
• Gene Symbol: RPL18A
• Gene ID (NCBI): 6142
• RRID: AB_3671508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q02543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.