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RPL19 Polyclonal antibody

RPL19 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14701-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

60S ribosomal protein L19, Large ribosomal subunit protein eL19, ribosomal protein L19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse spleen tissue, mouse testis tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

14701-1-AP targets RPL19 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6371

Product name: Recombinant human RPL19 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-196 aa of BC066315

Sequence: MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK

Predict reactive species
Full Name ribosomal protein L19
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066315
Gene Symbol RPL19
Gene ID (NCBI) 6143
RRIDAB_2181587
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP84098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.

Protocols

Product Specific Protocols
WB protocol for RPL19 antibody 14701-1-APDownload protocol
IHC protocol for RPL19 antibody 14701-1-APDownload protocol
IF protocol for RPL19 antibody 14701-1-APDownload protocol
IP protocol for RPL19 antibody 14701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
mouseWB

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
humanWB

Hum Mutat

Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia.

Authors - Gazda Hanna T HT

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans
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