RPL19 Polyclonal antibody

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• RPL19 Polyclonal antibody (14701-1-AP)

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• KD/KO Validated

RPL19 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14701-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

60S ribosomal protein L19, Large ribosomal subunit protein eL19, ribosomal protein L19

• 

  WB analysis of HeLa using 14701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 14701-1-AP

  WB result of RPL19 antibody (14701-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL19 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 14701-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 14701-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 14701-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 14701-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 14701-1-AP

  IP result of anti-RPL19 (IP:14701-1-AP, 4ug; Detection:14701-1-AP 1:3000) with HeLa cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 14701-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14701-1-AP (RPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 14701-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RPL19 (14701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, mouse spleen tissue, mouse testis tissue, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14701-1-AP targets RPL19 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6371

  Product name: Recombinant human RPL19 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-196 aa of BC066315

  Sequence: MSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLIKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGIGKRKGTANARMPEKVTWMRRMRILRRLLRRYRESKKIDRHMYHSLYLKVKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRSKTKEARKRREERLQAKKEEIIKTLSKEEETKK

• Full Name: ribosomal protein L19
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC066315
• Gene Symbol: RPL19
• Gene ID (NCBI): 6143
• RRID: AB_2181587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P84098
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.

Publications

What published studies show

The RPL26 antibody (catalog #14701-1-AP) has 10 total citations published in journals including Science Advances, EMBO Journal, Developmental Cell, Current Biology, Cell Reports, Phytomedicine, International Journal of Biological Macromolecules, Translational Research, and Human Mutation. Research fields span ribosome biology, cancer (colorectal cancer, glioblastoma, myeloma), neurodegeneration (tauopathy), spermiogenesis, meiosis, and Diamond-Blackfan anemia. Applications include Western blot and immunofluorescence in human and mouse samples.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells; Authors - Chandreyee Datta
		Phytomedicine; FBXO11 enhances colorectal cancer progression through TMBIM6 stabilization: Echinacoside as a natural inhibitor.; Authors - Chenghai He
		EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Int J Biol Macromol; Translational hub ribosomal protein S5 promotes glioblastoma progression by affecting translation patterns.; Authors - Lin Wang
mouse	WB	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans