RPL23 Recombinant monoclonal antibody, PBS Only

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• RPL23 Recombinant monoclonal antibody, PBS Only (86520-1-PBS)

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  WB analysis using 86520-1-RR (same clone as 86520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86520-1-RR (RPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 86520-1-RR (same clone as 86520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86520-1-RR (RPL23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86520-1-RR (same clone as 86520-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL23 antibody (86520-1-RR, Clone: 251372C7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86520-1-PBS in a different storage buffer formulation.

RPL23 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86520-1-PBS

Clone No.251372C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Large ribosomal subunit protein uL14, ribosomal protein L23, rpL17

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Product Information

86520-1-PBS targets RPL23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9120

  Product name: Recombinant human RPL23 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-140 aa of BC010114

  Sequence: MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA

• Full Name: ribosomal protein L23
• Calculated Molecular Weight: 140 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC010114
• Gene Symbol: RPL23
• Gene ID (NCBI): 9349
• RRID: AB_3744942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62829
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].