RPL23 Recombinant monoclonal antibody

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  WB analysis using 86520-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86520-1-RR (RPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86520-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPL23 antibody (86520-1-RR, Clone: 251372C7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPL23 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86520-1-RR

Clone No.251372C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Large ribosomal subunit protein uL14, ribosomal protein L23, rpL17

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, BxPC-3 cells, NIH/3T3 cells, PC-12 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86520-1-RR targets RPL23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9120

  Product name: Recombinant human RPL23 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-140 aa of BC010114

  Sequence: MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA

• Full Name: ribosomal protein L23
• Calculated Molecular Weight: 140 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC010114
• Gene Symbol: RPL23
• Gene ID (NCBI): 9349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62829
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.