RPL23A Polyclonal antibody

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• RPL23A Polyclonal antibody (16386-1-AP)

RPL23A Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16386-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

60S ribosomal protein L23a, Large ribosomal subunit protein uL23, MDA20, ribosomal protein L23a

• 

  WB analysis using 16386-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16386-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16386-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 16386-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16386-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 16386-1-AP

  IP result of anti-RPL23A (IP:16386-1-AP, 4ug; Detection:16386-1-AP 1:500) with HepG2 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 16386-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16386-1-AP (RPL23A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 16386-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16386-1-AP (RPL23A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue, human heart tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16386-1-AP targets RPL23A in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9424

  Product name: Recombinant human RPL23A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-156 aa of BC014459

  Sequence: MAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII

• Full Name: ribosomal protein L23a
• Calculated Molecular Weight: 156 aa, 18 kDa
• Observed Molecular Weight: 18-23 kDa
• GenBank Accession Number: BC014459
• Gene Symbol: RPL23A
• Gene ID (NCBI): 6147
• RRID: AB_2269755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL23A Polyclonal antibody (Cat# 16386-1-AP) has 11 citations published in journals including Cell Stem Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, Brain, Cell Death & Disease, Nature Structural & Molecular Biology, Cell Reports, Breast Cancer Research, and Gene. Associated research fields encompass ribosome biogenesis and assembly, stem cell regeneration, RNA processing and surveillance, cancer progression (colorectal and breast), neurodegeneration, viral gene expression, and neural development.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
mouse	WB,RIP	Nat Commun; The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis.; Authors - Mehdi Pirouz
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
human	WB	Mol Cell; An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation; Authors - Jong-Sun Lee
		Nucleic Acids Res; Stable Cas9 expression regulates cell growth by facilitating mTORC2 activation; Authors - Le Yu
human	WB	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič