RPL27A Polyclonal antibody, PBS Only

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• RPL27A Polyclonal antibody, PBS Only (16002-1-PBS)

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  WB analysis of A549 using 16002-1-AP (same clone as 16002-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 16002-1-AP (same clone as 16002-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 16002-1-AP (same clone as 16002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 16002-1-AP (same clone as 16002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 16002-1-AP (same clone as 16002-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RPL27A antibody (16002-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16002-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RPL27A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16002-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

60S ribosomal protein L27a, Large ribosomal subunit protein uL15, ribosomal protein L27a

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Product Information

16002-1-PBS targets RPL27A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8596

  Product name: Recombinant human RPL27A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-148 aa of BC005326

  Sequence: MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGACVLVA

• Full Name: ribosomal protein L27a
• Calculated Molecular Weight: 148 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC005326
• Gene Symbol: RPL27A
• Gene ID (NCBI): 6157
• RRID: AB_2878206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46776
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.