RPL28 Polyclonal antibody

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• RPL28 Polyclonal antibody (16649-1-AP)

RPL28 Polyclonal Antibody for WB, ELISA

Cat No. 16649-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

ribosomal protein L28, Large ribosomal subunit protein eL28, 60S ribosomal protein L28

• 

  WB analysis of HeLa using 16649-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16649-1-AP (RPL28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 16649-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16649-1-AP (RPL28 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 16649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16649-1-AP (RPL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse ovary using 16649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16649-1-AP (RPL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse ovary using 16649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16649-1-AP (RPL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16649-1-AP targets RPL28 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10036

  Product name: Recombinant human RPL28 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-137 aa of BC010173

  Sequence: MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVIKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS

• Full Name: ribosomal protein L28
• Calculated Molecular Weight: 137 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC010173
• Gene Symbol: RPL28
• Gene ID (NCBI): 6158
• RRID: AB_2254021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46779
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL28 Polyclonal antibody (Cat# 16649-1-AP) has 9 citations published in journals including Mol Cell, Genes Dev, Aging Cell, J Pineal Res, Transl Res, Sci Rep, iScience (×2), and eLife. Research fields span mitochondrial metabolism and redox rescue, ribosome biogenesis and cellular senescence, radiation-induced tissue damage, Shwachman-Diamond syndrome, microvesicle biology in myeloma, colorectal cancer prognosis, spermatogenesis, leukemia cell survival in bone marrow, and cerebellar translation regulation.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Reversal of mitochondrial malate dehydrogenase 2 enables anaplerosis via redox rescue in respiration-deficient cells; Authors - Patricia Altea-Manzano
human	WB	Aging Cell; Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence.; Authors - Asimina Pantazi
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
mouse	WB	Genes Dev; Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.; Authors - Andrew J Finch
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
		Sci Rep; Germline variability and tumor expression level of ribosomal protein gene RPL28 are associated with survival of metastatic colorectal cancer patients.; Authors - Adrien Labriet