RPL29 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83377-1-RR (same clone as 83377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83377-1-RR (same clone as 83377-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83377-1-RR (same clone as 83377-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83377-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83377-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83377-1-RR against Human RPL29 were performed. The affinity constant is 0.254 nM.

RPL29 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83377-1-PBS

Clone No.240062F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240062F9, 60S ribosomal protein L29, Cell surface heparin-binding protein HIP, HIP, HUMRPL29

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Product Information

83377-1-PBS targets RPL29 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34100

  Product name: Recombinant human RPL29 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-97 aa of BC008926

  Sequence: MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLA

• Full Name: ribosomal protein L29
• Calculated Molecular Weight: 159 aa, 18 kDa
• GenBank Accession Number: BC008926
• Gene Symbol: RPL29
• Gene ID (NCBI): 6159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P47914
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).