Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15387-1-AP targets RPL6 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6840 Product name: Recombinant human RPL6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-288 aa of BC004138 Sequence: MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF Predict reactive species |
| Full Name | ribosomal protein L6 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC004138 |
| Gene Symbol | RPL6 |
| Gene ID (NCBI) | 6128 |
| RRID | AB_2182042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02878 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RPL6 antibody 15387-1-AP | Download protocol |
| IF protocol for RPL6 antibody 15387-1-AP | Download protocol |
| IHC protocol for RPL6 antibody 15387-1-AP | Download protocol |
| WB protocol for RPL6 antibody 15387-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RPL6 Polyclonal antibody (Cat# 15387-1-AP) has 12 citations published in journals including Cell Research, Cell Discovery, Nucleic Acids Research, Journal of Experimental & Clinical Cancer Research, Journal of Pineal Research, Journal of Ethnopharmacology, Molecular & Cellular Proteomics, Translational Research, Journal of Cell Biology, Cancer Science, iScience, and Neurochemistry Research. Research fields span ribosome biology, DNA damage repair, cancer (leukemia, glioblastoma, breast cancer), exosomes, epilepsy, and neuronal protection.
| Species | Application | Title |
|---|---|---|
Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. | ||
Nucleic Acids Res ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation | ||
J Exp Clin Cancer Res PSMB10 maintains the stemness of chemotherapeutic drug-resistant leukemia cells by inhibiting senescence and cytotoxic T lymphocyte-mediated killing in a ubiquitinated degradation manner | ||
J Pineal Res Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin | ||
J Ethnopharmacol Plasma exosomes proteome profiling discovers protein markers associated with the therapeutic effect of Chaihu-Longgu-Muli decoction on temporal lobe epilepsy |
