RPL7L1 Polyclonal antibody

• 

  WB analysis using 16707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16707-1-AP

  IP result of anti-RPL7L1 (IP:16707-1-AP, 4ug; Detection:16707-1-AP 1:1000) with HepG2 cells lysate 2240 ug.

• 

  IHC staining of human liver cancer using 16707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 16707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of MCF-7 using 16707-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPL7L1 antibody 16707-1-AP at 1:25 dillution and Rhodamine-labeled goat anti-rabbit IgG (red).

RPL7L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16707-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RPL7 L1, Ribosomal protein uL30-like, Large ribosomal subunit protein uL30-like 1, 60S ribosomal protein L7-like 1

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16707-1-AP targets RPL7L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10106

  Product name: Recombinant human RPL7L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-246 aa of BC058020

  Sequence: MAEQEQRKIPLVPENLLKKRKAYQALKATQAKQALLAKKEQKKGKGLRFKRLESFLHDSWRQKRDKVRLRRLEVKPHALELPDKHSLAFVVRIERIDGVSLLVQRTIARLRLKKIFSGVFVKVTPQNLKMLRIVEPYVTWGFPNLKSVRELILKRGQAKVKNKTIPLTDNTVIEEHLGKFGVICLEDLIHGIAFPGKHFQEISWFLCPFHLSVARHATKNRVGFLKEMGTPGYRGERINQLIRQLN

• Full Name: ribosomal protein L7-like 1
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC058020
• Gene Symbol: RPL7L1
• Gene ID (NCBI): 285855
• RRID: AB_1851609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6DKI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
mouse		Zygote; Identification of four genes required for mammalian blastocyst formation.; Authors - Maserati Marc M