Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 16707-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 16707-1-AP
IP result of anti-RPL7L1 (IP:16707-1-AP, 4ug; Detection:16707-1-AP 1:1000) with HepG2 cells lysate 2240 ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected in
HepG2 cells
Positive IHC detected in
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:16000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 16707-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 16707-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 16707-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 40x lens).
IP Figures
IP experiment of HepG2 using 16707-1-AP
IP result of anti-RPL7L1 (IP:16707-1-AP, 4ug; Detection:16707-1-AP 1:1000) with HepG2 cells lysate 2240 ug.
IF/ICC Figures
IF Staining of MCF-7 using 16707-1-AP
Immunofluorescent analysis of MCF-7 cells, using RPL7L1 antibody 16707-1-AP at 1:25 dillution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RPL7L1 Polyclonal antibody
Catalog Number
16707-1-AP
Citations
3
Dilutions
WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
