RPL7L1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• RPL7L1 Polyclonal antibody (16707-1-AP)

RPL7L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16707-1-AP Copied!

Publications (3)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RPL7 L1, Ribosomal protein uL30-like, Large ribosomal subunit protein uL30-like 1, 60S ribosomal protein L7-like 1

• 

  WB analysis using 16707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16707-1-AP (RPL7L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 16707-1-AP

  IP result of anti-RPL7L1 (IP:16707-1-AP, 4ug; Detection:16707-1-AP 1:1000) with HepG2 cells lysate 2240 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16707-1-AP (RPL7L1 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 16707-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPL7L1 antibody 16707-1-AP at 1:25 dillution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RPL7L1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16707-1-AP targets RPL7L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10106

  Product name: Recombinant human RPL7L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-246 aa of BC058020

  Sequence: MAEQEQRKIPLVPENLLKKRKAYQALKATQAKQALLAKKEQKKGKGLRFKRLESFLHDSWRQKRDKVRLRRLEVKPHALELPDKHSLAFVVRIERIDGVSLLVQRTIARLRLKKIFSGVFVKVTPQNLKMLRIVEPYVTWGFPNLKSVRELILKRGQAKVKNKTIPLTDNTVIEEHLGKFGVICLEDLIHGIAFPGKHFQEISWFLCPFHLSVARHATKNRVGFLKEMGTPGYRGERINQLIRQLN

• Full Name: ribosomal protein L7-like 1
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC058020
• Gene Symbol: RPL7L1
• Gene ID (NCBI): 285855
• RRID: AB_1851609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6DKI1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL7L1 Polyclonal antibody (Cat# 16707-1-AP) has 3 total citations published in iScience, Biology (Basel), and Zygote. The associated research fields include pluripotent stem cell gene regulation, TIA1 protein interactome mapping, and mammalian blastocyst development.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
mouse		Zygote; Identification of four genes required for mammalian blastocyst formation.; Authors - Maserati Marc M