RPL9 Monoclonal antibody

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  WB analysis using 68054-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of mouse cerebellum using 68054-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 68054-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 68054-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse cerebellum using 68054-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), FUS/TLS antibody (11570-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 68054-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

RPL9 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 68054-1-Ig

Clone No.2A6B11

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

2A6B11, 60S ribosomal protein L9, FLJ27456, Large ribosomal subunit protein uL6, OK/SW-cl.103

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, A549 cells, HepG2 cells, MCF-7 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68054-1-Ig targets RPL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5391

  Product name: Recombinant human RPL9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-192 aa of BC066318

  Sequence: MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQADE

• Full Name: ribosomal protein L9
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC066318
• Gene Symbol: RPL9
• Gene ID (NCBI): 6133
• RRID: AB_2918795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P32969
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	J Cardiovasc Transl Res; CREB1 Silencing Protects Against Inflammatory Response in Rats with Deep Vein Thrombosis Through Reducing RPL9 Expression and Blocking NF-κB Signaling; Authors - Xiaorong Jian