RPL9 Monoclonal antibody, PBS Only

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  WB analysis using 68054-1-Ig (same clone as 68054-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), FUS/TLS antibody (11570-1-AP, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

RPL9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 68054-1-PBS

Clone No.2A6B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

2A6B11, 60S ribosomal protein L9, FLJ27456, Large ribosomal subunit protein uL6, OK/SW-cl.103

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Product Information

68054-1-PBS targets RPL9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5391

  Product name: Recombinant human RPL9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-192 aa of BC066318

  Sequence: MKTILSNQTVDIPENVDITLKGRTVIVKGPRGTLRRDFNHINVELSLLGKKKKRLRVDKWWGNRKELATVRTICSHVQNMIKGVTLGFRYKMRSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQADE

• Full Name: ribosomal protein L9
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC066318
• Gene Symbol: RPL9
• Gene ID (NCBI): 6133
• RRID: AB_2918795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P32969
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.