RPLP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPLP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21636-1-AP

Synonyms

P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 21636-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21636-1-AP (RPLP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21636-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21636-1-AP (RPLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 21636-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21636-1-AP (RPLP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21636-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21636-1-AP (RPLP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21636-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPLP1 antibody (21636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

21636-1-AP targets RPLP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPLP1 fusion protein Ag16268
• Full Name: ribosomal protein, large, P1
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC003369
• Gene Symbol: RPLP1
• Gene ID (NCBI): 6176
• RRID: AB_2878894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa.

Publications

Species	Application	Title
pig	WB	Virulence; RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.; Authors - Longxiang Zhang ; KD Validated
mouse	WB	Mol Hum Reprod; 60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro.; Authors - Zahraa Alali
human	WB	Int J Mol Sci; RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro; Authors - Riley Peterson
human	WB,IHC	Am J Cancer Res; Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma; Authors - Xinhui Du
human	WB,IF	Nat Commun; RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR; Authors - Weijing Yang