RPN2 Recombinant monoclonal antibody

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• RPN2 Recombinant monoclonal antibody (88014-2-RR)

• 

  WB analysis using 88014-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88014-2-RR (RPN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RPN2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88014-2-RR

Clone No.260152B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, RIBIIR, Ribophorin 2, Ribophorin-2

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Tested Applications

• Positive WB detected in: HepG2 cells, SK-BR-3 cells, Caco-2 cells, Calu-3 cells, BxPC-3 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88014-2-RR targets RPN2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag40161

  Product name: Recombinant human RPN2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 222-452 aa of BC003560

  Sequence: VGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAE

• Full Name: ribophorin II
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68-69 kDa
• GenBank Accession Number: BC003560
• Gene Symbol: RPN2
• Gene ID (NCBI): 6185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04844
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPN2 (Ribophorin II), an integral rough ER membrane glycoprotein, is part of an N-oligosaccharyl transferase complex and is involved in translocation and the maintenance of the structural uniqueness of the rough ER. RPN2 expression has been reported to be significantly associated with solid cancers, including breast cancer, esophageal squamous cell carcinoma, colorectal cancer, and lung squamous cell carcinoma.