HeLa cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15461-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
MCF7 cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15461-1-AP
IP result of anti-RPP25 (IP:15461-1-AP, 4ug; Detection:15461-1-AP 1:300) with HeLa cells lysate 2800 ug.
IP result of anti-RPP25 (IP:15461-1-AP, 4ug; Detection:15461-1-AP 1:300) with HeLa cells lysate 2800 ug.
IHC staining of human cervical cancer using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human small intestine using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 15461-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPP25 antibody (15461-1-AP) at dilution of 1:600 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPP25 antibody (15461-1-AP) at dilution of 1:600 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human small intestine tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA.
HeLa cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15461-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15461-1-AP (RPP25 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human cervical cancer using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human small intestine using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 15461-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15461-1-AP (RPP25 Antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of HeLa using 15461-1-AP
IP result of anti-RPP25 (IP:15461-1-AP, 4ug; Detection:15461-1-AP 1:300) with HeLa cells lysate 2800 ug.
IF/ICC Figures
IF Staining of HeLa using 15461-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPP25 antibody (15461-1-AP) at dilution of 1:600 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RPP25 Polyclonal antibody
Catalog Number
15461-1-AP
Citations
1
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:300-1:1200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
