RPP30 Polyclonal antibody, PBS Only

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• RPP30 Polyclonal antibody, PBS Only (18435-1-PBS)

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  IHC staining of human lung cancer using 18435-1-AP (same clone as 18435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18435-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 18435-1-AP (same clone as 18435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18435-1-AP (RPP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18435-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 18435-1-AP (same clone as 18435-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (18435-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18435-1-PBS in a different storage buffer formulation.

RPP30 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 18435-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2

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Product Information

18435-1-PBS targets RPP30 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8824

  Product name: Recombinant human RPP30 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-268 aa of BC006991

  Sequence: MAVFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG

• Full Name: ribonuclease P/MRP 30kDa subunit
• Calculated Molecular Weight: 268 aa, 29 kDa
• GenBank Accession Number: BC006991
• Gene Symbol: RPP30
• Gene ID (NCBI): 10556
• RRID: AB_3669300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78346
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPP30 is part of the polyribonuclease P complex and the ribonuclease MRP complex, and is primarily involved in the 5' end maturation of tRNA, and may also be involved in other RNA metabolic processes, including RNA synthesis, processing, and maturation. RPP30 is widely expressed in a variety of tissues, including testis, lymph nodes, etc., and is also a potential biomarker for the diagnosis and prognosis of gastric cancer.