RPS16 Polyclonal antibody

RPS16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15603-1-AP

Synonyms

Small ribosomal subunit protein uS9, ribosomal protein S16, 40S ribosomal protein S16

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Validation Data Gallery

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  WB analysis of HeLa using 15603-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15603-1-AP (RPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15603-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15603-1-AP (RPS16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15603-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15603-1-AP (RPS16 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15603-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15603-1-AP (RPS16 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 15603-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPS16 antibody 15603-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

15603-1-AP targets RPS16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS16 fusion protein Ag7986
• Full Name: ribosomal protein S16
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC004324
• Gene Symbol: RPS16
• Gene ID (NCBI): 6217
• RRID: AB_2180168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS16, the 40S ribosmal protein S16, belongs to the ribosomal protein S9P family. It is located in the cytoplasm and can be acetylated. RPS16 is extensively involved in rRNA processing, translational elongation, initiation and termination via its RNA binding activity. Catalog#15603-1-AP is a rabbit polyclonal antibody raised against full-length RPS16 of human origin.

Publications

Species	Application	Title
human	WB	Sci Signal; Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas; Authors - Matthew J Knarr
human	WB	Proteomics Clin Appl; New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.; Authors - Huang Rui R
human	IHC	Int J Biol Markers; Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation; Authors - Lida Suo