RPS17 Recombinant monoclonal antibody

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  WB analysis using 83848-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83848-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83848-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83848-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

RPS17 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83848-4-RR

Clone No.240851H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240851H3, 40S ribosomal protein S17, DBA4, ribosomal protein S17, RPS17L

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Jurkat cells, HEK-293 cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83848-4-RR targets RPS17 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9334

  Product name: Recombinant human RPS17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-135 aa of BC009407

  Sequence: MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV

• Full Name: ribosomal protein S17
• Calculated Molecular Weight: 135 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC009407
• Gene Symbol: RPS17
• Gene ID (NCBI): 6218
• RRID: AB_3671434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08708
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.