RPS21 Polyclonal antibody

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16946-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 16946-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16946-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS21 antibody (16946-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

RPS21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16946-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Small ribosomal subunit protein eS21, ribosomal protein S21, 40S ribosomal protein S21

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse liver tissue, SGC-7901 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

16946-1-AP targets RPS21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10557

  Product name: Recombinant human RPS21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-81 aa of BC018140

  Sequence: MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSK

• Full Name: ribosomal protein S21
• Calculated Molecular Weight: 81 aa, 9 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC018140
• Gene Symbol: RPS21
• Gene ID (NCBI): 6227
• RRID: AB_2180351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P63220
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Endocrinology; Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice; Authors - Abraham Behrmann
		J Cell Sci; Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.; Authors - Qiancong Zhao
human	IHC	Oncol Lett; Identification of the key genes and pathways in prostate cancer.; Authors - Shutong Fan
human	WB,IHC	Med Oncol; Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.; Authors - Zumu Liang ; KD Validated
mouse	IHC	Reprod Sci; Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes; Authors - Natsuki Nakanishi