Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver tissue, SGC-7901 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16946-1-AP targets RPS21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10557 Product name: Recombinant human RPS21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC018140 Sequence: MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSK Predict reactive species |
| Full Name | ribosomal protein S21 |
| Calculated Molecular Weight | 81 aa, 9 kDa |
| Observed Molecular Weight | 9 kDa |
| GenBank Accession Number | BC018140 |
| Gene Symbol | RPS21 |
| Gene ID (NCBI) | 6227 |
| RRID | AB_2180351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63220 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS21 antibody 16946-1-AP | Download protocol |
| IHC protocol for RPS21 antibody 16946-1-AP | Download protocol |
| WB protocol for RPS21 antibody 16946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS21 Polyclonal antibody (Cat# 16946-1-AP) has 6 total citations published in Oncogene, Med Oncol, J Cell Sci, Endocrinology, Reprod Sci, and Oncol Lett. Research fields span lung adenocarcinoma and protein translation, prostate cancer biomarker identification, ribosomal gene transcription in embryonic heart development, Wnt-mediated vascular smooth muscle function, and ribosomal pathway dysregulation in polycystic ovary syndrome.
| Species | Application | Title |
|---|---|---|
Oncogene HERC4 synergizes with USP16 to promote lung adenocarcinoma growth by augmenting protein translation via stabilizing RPS15. | ||
Med Oncol Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.
| ||
Endocrinology Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice | ||
Reprod Sci Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes | ||
