RPS23 Polyclonal antibody

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  WB analysis using 29834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29834-1-AP (RPS23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 29834-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS23 antibody (29834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPS23 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29834-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: A431 cells, U2OS cells, human placenta tissue, mouse ovary tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29834-1-AP targets RPS23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS23 fusion protein Ag31190
• Full Name: ribosomal protein S23
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: NM_001025
• Gene Symbol: RPS23
• Gene ID (NCBI): 6228
• RRID: AB_3669698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62266
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS23 (40S ribosomal protein S23), also called small ribosomal subunit protein uS12, is a subunit of the 40S ribosome and the first precursor of the small eukaryotic ribosomal subunit (PMID: 34516797). It is positioned in the decoding center of the ribosome that serves to maintain translational fidelity by monitoring the complementarity between the mRNA codons being translated and the anti-codons of aminoacyl-tRNAs (PMID: 28257692; 23636399).