RPS23 Polyclonal antibody

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• RPS23 Polyclonal antibody (29834-1-AP)

RPS23 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 29834-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Small ribosomal subunit protein uS12

• 

  WB analysis using 29834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29834-1-AP (RPS23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 29834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29834-1-AP (RPS23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 29834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29834-1-AP (RPS23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 29834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29834-1-AP (RPS23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 29834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29834-1-AP (RPS23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 29834-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29834-1-AP (RPS23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 29834-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS23 antibody (29834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, U2OS cells, human placenta tissue, mouse ovary tissue
• Positive IHC detected in: human lung cancer tissue, human stomach cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29834-1-AP targets RPS23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31190

  Product name: Recombinant human RPS23 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2-143 aa of NM_001025

  Sequence: GKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS

• Full Name: ribosomal protein S23
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: NM_001025
• Gene Symbol: RPS23
• Gene ID (NCBI): 6228
• RRID: AB_3669698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62266
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS23 (40S ribosomal protein S23), also called small ribosomal subunit protein uS12, is a subunit of the 40S ribosome and the first precursor of the small eukaryotic ribosomal subunit (PMID: 34516797). It is positioned in the decoding center of the ribosome that serves to maintain translational fidelity by monitoring the complementarity between the mRNA codons being translated and the anti-codons of aminoacyl-tRNAs (PMID: 28257692; 23636399).

Publications

What published studies show

The RPS23 Polyclonal antibody (Cat# 29834-1-AP) has 1 total citation. It was cited in Journal of Translational Medicine (IF 9.7), in a 2025 study investigating how paternal ribosome biogenesis dysfunction induced by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. The associated research fields include reproductive biology, ribosome biogenesis, and developmental toxicology. The antibody was used for Western blot in mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Transl Med; Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice.; Authors - Xin Li