RPS24 Polyclonal antibody

RPS24 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14831-1-AP

Synonyms

40S ribosomal protein S24, DBA3, ribosomal protein S24, RPS24

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 14831-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14831-1-AP (RPS24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14831-1-AP (RPS24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14831-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14831-1-AP (RPS24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 14831-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 14831-1-AP (RPS24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, HL-60 cells
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14831-1-AP targets RPS24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS24 fusion protein Ag6621
• Full Name: ribosomal protein S24
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC000523
• Gene Symbol: RPS24
• Gene ID (NCBI): 6229
• RRID: AB_2878084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

40S ribosomal protein S24 (RPS24) is a 133 amino acid protein, which belongs to the ribosomal protein S24e family. RPS24 is required for processing of pre-rRNA and maturation of 40S ribosomal subunits. RPS24 has an association with Diamond-Blackfan anemia 3.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; ZEA and DON inhibited inflammation after L. monocytogenes infection and induced ribosomal hyperfunction.; Authors - Guodong Cai
human	IHC	Int J Mol Sci; RPS24 Is Associated with a Poor Prognosis and Immune Infiltration in Hepatocellular Carcinoma; Authors - Haiyuan Li
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao