Tested Applications
| Positive WB detected in | mouse ovary tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14909-1-AP targets RPS26 in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6706 Product name: Recombinant human RPS26 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-115 aa of BC002604 Sequence: MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCAIHSKVVRNRSREARKDRTPPPRFRPAGAAPRPPPKPM Predict reactive species |
| Full Name | ribosomal protein S26 |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 18-21 kDa |
| GenBank Accession Number | BC002604 |
| Gene Symbol | RPS26 |
| Gene ID (NCBI) | 6231 |
| RRID | AB_2180361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62854 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IHC protocol for RPS26 antibody 14909-1-AP | Download protocol |
| IP protocol for RPS26 antibody 14909-1-AP | Download protocol |
| WB protocol for RPS26 antibody 14909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS26 antibody (Cat# 14909-1-AP) has 17 total citations published in journals including Nature Communications, Molecular Cell, Nucleic Acids Research, PNAS, Brain, Oncogene, Cell Death & Disease, Journal of Experimental & Clinical Cancer Research, Cellular and Molecular Life Sciences, International Journal of Molecular Sciences, Toxics, eLife, and bioRxiv. Research fields span ribosome biology, translational control, cancer, neurodegeneration, oocyte development, immune signaling, and proteomics.
| Species | Application | Title |
|---|---|---|
Nat Commun Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity. | ||
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Nucleic Acids Res The intrinsic properties of mRNAs define their translation efficiency at ribosome shortage.
| ||
J Exp Clin Cancer Res Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression. | ||
Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation |
Reviews
What customers say
The single review for 14909-1-AP is a 5-star rating from September 2023. The reviewer used the antibody for Western Blot on an esophageal cancer cell line at a dilution of 1:1500 (lot 00013757) and reported that it works very stably, with effective dilutions ranging from 1:1000 to 1:2000. Overall, the feedback is positive, highlighting consistent performance and flexibility in working dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shinford (Verified Customer) (09-24-2023) | The esophageal cancer cell line is used. The antibody works very stably, and the concentration can be from 1:1000 to 1:2000.
 |
