CoraLite®594-conjugated RPS3 Monoclonal antibody

• Featured Product
• KD/KO Validated

RPS3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G7H4

Cat no : CL594-66046

Synonyms

40S ribosomal protein S3, FLJ26283, FLJ27450, ribosomal protein S3, Ribosome Marker, RPS3

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66046

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66046 (RPS3 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL594-66046

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated RPS3 antibody (CL594-66046, Clone: 2G7H4 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66046 is RPS3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPS3 fusion protein Ag7515
• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC003137
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• RRID: AB_2883491
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.