RPS3A Recombinant monoclonal antibody

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• RPS3A Recombinant monoclonal antibody (86254-3-RR)

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  WB analysis using 86254-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86254-3-RR (RPS3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of Human lung cancer （Adenocarcinoma） using 86254-3-RR

  Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 86254-3-RR (RPS3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human lung cancer （Adenocarcinoma） using 86254-3-RR

  Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 86254-3-RR (RPS3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 86254-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (86254-3-RR, Clone: 251033B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPS3A Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86254-3-RR

Clone No.251033B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Raji cells, A431 cells, HEK-293T cells, NIH/3T3 cells, PC-12 cells
• Positive IHC detected in: Human lung cancer （Adenocarcinoma）; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86254-3-RR targets RPS3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5273

  Product name: Recombinant human RPS3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-264 aa of BC066926

  Sequence: MAVSKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV

• Full Name: ribosomal protein S3A
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC066926
• Gene Symbol: RPS3A
• Gene ID (NCBI): 6189
• RRID: AB_3744755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61247
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.