RPS4X Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85040-5-RR (same clone as 85040-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85040-5-RR (same clone as 85040-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS4X antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85040-5-RR (same clone as 85040-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85040-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

RPS4X Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 85040-5-PBS

Clone No.242697E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

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Product Information

85040-5-PBS targets RPS4X in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6513

  Product name: Recombinant human SCAR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-263 aa of BC000472

  Sequence: MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG

• Full Name: ribosomal protein S4, X-linked
• Calculated Molecular Weight: 263 aa, 30 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC000472
• Gene Symbol: RPS4X
• Gene ID (NCBI): 6191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62701
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)