RPS4X Recombinant antibody

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  WB analysis using 85040-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 85040-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SCAR antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 85040-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85040-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

RPS4X Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 85040-5-RR

Clone No.242697E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

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Tested Applications

• Positive WB detected in: HT-29 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse spleen tissue, mouse brain tissue, rat liver tissue
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85040-5-RR targets RPS4X in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPS4X fusion protein Ag6513
• Full Name: ribosomal protein S4, X-linked
• Calculated Molecular Weight: 263 aa, 30 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC000472
• Gene Symbol: RPS4X
• Gene ID (NCBI): 6191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62701
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)