RPS6 Polyclonal antibody

RPS6 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 14823-1-AP

Synonyms

40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, mouse kidney tissue
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14823-1-AP targets RPS6 in WB, IP, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS6 fusion protein Ag6599
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.It's molecular weight is about 29kd.

Protocols

Product Specific Protocols
WB protocol for RPS6 antibody 14823-1-APDownload protocol
IP protocol for RPS6 antibody 14823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Ribosome 18S m6A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.

Authors - Bowen Rong
humanWB,FC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
ratWB

Mol Med Rep

Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy.

Authors - Zhongzhong Liu
humanWB,IF

FEBS Lett

Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.

Authors - Siyue Qin
humanWB

Cell Commun Signal

Bifunctional enzyme ATIC promotes propagation of hepatocellular carcinoma by regulating AMPK-mTOR-S6 K1 signaling.

Authors - Minjing Li
mouseWB

Cell Death Dis

Macrophages confer resistance to PI3K inhibitor GDC-0941 in breast cancer through the activation of NF-κB signaling.

Authors - Muhammad Waqas Usman

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band as predicted around 30 kDa.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart