Validation Data Gallery
|Positive WB detected in||K-562 cells, MCF-7 cells, mouse kidney tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14823-1-AP targets S6 Ribosomal protein in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S6 Ribosomal protein fusion protein Ag6599|
|Full Name||ribosomal protein S6|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||29-32 kDa|
|GenBank accession number||BC000524|
|Gene ID (NCBI)||6194|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
|Product Specific Protocols|
|WB protocol for S6 Ribosomal protein antibody 14823-1-AP||Download protocol|
|IHC protocol for S6 Ribosomal protein antibody 14823-1-AP||Download protocol|
|IP protocol for S6 Ribosomal protein antibody 14823-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (01-30-2020)
Strong, clear band as predicted around 30 kDa.