S6 Ribosomal protein Monoclonal antibody
S6 Ribosomal protein Monoclonal Antibody for FC, IF, WB, ELISA
Cat no : 66886-1-Ig
|Positive WB detected in||HeLa cells, A431 cells, NIH/3T3 cells, rat liver tissue, HEEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, HEK-293 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66886-1-Ig targets S6 Ribosomal protein in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||S6 Ribosomal protein fusion protein Ag6599|
|Full Name||ribosomal protein S6|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||29-32 kDa|
|GenBank accession number||BC000524|
|Gene ID (NCBI)||6194|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
|Product Specific Protocols|
|WB protocol for S6 Ribosomal protein antibody 66886-1-Ig||Download protocol|
|IF protocol for S6 Ribosomal protein antibody 66886-1-Ig||Download protocol|
|FC protocol for S6 Ribosomal protein antibody 66886-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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