S6 Ribosomal protein Monoclonal antibody

S6 Ribosomal protein Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C3E10

Cat no : 66886-1-Ig

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33



Tested Applications

Positive WB detected inHeLa cells, A431 cells, NIH/3T3 cells, rat liver tissue, HEEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, HEK-293 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66886-1-Ig targets S6 Ribosomal protein in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S6 Ribosomal protein fusion protein Ag6599
Full Name ribosomal protein S6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2882218
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
WB protocol for S6 Ribosomal protein antibody 66886-1-IgDownload protocol
IF protocol for S6 Ribosomal protein antibody 66886-1-IgDownload protocol
FC protocol for S6 Ribosomal protein antibody 66886-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genome Biol

A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.

Authors - Sooncheol Lee
humanWB,FC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
humanIF

Microbiol Spectr

Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1.

Authors - Kaitao Zhao
mouseWB

Ecotoxicol Environ Saf

Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.

Authors - Yifan Hong
humanWB

Sci Rep

YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma.

Authors - Yuting Zhan
ratWB

Endocrinology

Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-Mediated Effects on mTORC1 and mTORC2.

Authors - Yan Fu