RPS6 Monoclonal antibody

RPS6 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3E10

Cat no : 66886-1-Ig

Synonyms

40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6



Tested Applications

Positive WB detected inA431 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66886-1-Ig targets RPS6 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RPS6 fusion protein Ag6599
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
WB protocol for RPS6 antibody 66886-1-IgDownload protocol
IF protocol for RPS6 antibody 66886-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genome Biol

A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.

Authors - Sooncheol Lee
ratWB

Endocrinology

Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-Mediated Effects on mTORC1 and mTORC2.

Authors - Yan Fu
humanIF

Microbiol Spectr

Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1.

Authors - Kaitao Zhao