S6 Ribosomal protein Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• S6 Ribosomal protein Recombinant monoclonal antibody (80208-1-RR)

S6 Ribosomal protein Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 80208-1-RR Copied!

Clone No. 6L9

Publications (21)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF/ICC, ELISA

Synonyms

RPS6, S6, 40S ribosomal protein S6, 6L9, OK/SW-cl.2

• 

  WB analysis using 80208-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 80208-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of zebrafish using 80208-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 80208-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for S6 Ribosomal protein in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: LNCaP cells, RAW 264.7 cells, zebrafish tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, 4T1 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80208-1-RR targets S6 Ribosomal protein in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_2918876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

What published studies show

The S6 Ribosomal protein Recombinant monoclonal antibody (Cat# 80208-1-RR) has 21 citations across journals including Nature Aging, Signal Transduction and Targeted Therapy, Oncogene, Cell Reports, Cancer Letters, Metabolism, Free Radical Biology and Medicine, Journal of Biological Chemistry, and ACS Omega. Research fields span hepatocellular carcinoma, neurodegeneration, Parkinson's disease, gastric cancer, colorectal cancer, osteoarthritis, osteoporosis, ferroptosis, pulmonary fibrosis, vascular injury, renal injury, liver injury, and radiation-induced lung injury, with WB and IF as primary applications.

Automatically generated from the 21 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Signal Transduct Target Ther; Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma; Authors - Minghao Xu
mouse	WB	Nat Aging; Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration; Authors - Liansheng Zhang
		J Adv Res; Targeting FBXO48 with BC1618 promotes axon regeneration and alleviates symptoms of Parkinson's disease.; Authors - Yong-Quan Sun
human, mouse	WB	Metabolism; ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake.; Authors - Dehui Hou
human	WB	Biomark Res; Deciphering metabolic heterogeneity in retinoblastoma unravels the role of monocarboxylate transporter 1 in tumor progression; Authors - Junjie Tang
human	WB	Cancer Lett; Hypoxia-driven phase separation of the PABP1/eIF4B complex forms stress granules and activates ChaC2 translation to promote polyunsaturated lipids-supported peritoneal metastasis in gastric cancer.; Authors - Zaihuan Lin