RPS6KL1 Polyclonal antibody

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  WB analysis of CHO using 24757-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24757-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24757-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 24757-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 24757-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 40x lens).

RPS6KL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24757-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, IHC, ELISA

Synonyms

RPS6KL1

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Tested Applications

• Positive WB detected in: CHO cells
• Positive IHC detected in: human testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24757-1-AP targets RPS6KL1 in WB, IHC, ELISA applications and shows reactivity with human, hamster samples.

• Tested Reactivity: human, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15099

  Product name: Recombinant human RPS6KL1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 168-518 aa of BC004540

  Sequence: YMTKLLRYFVSEDSIFLHLEHVQGGTLWSHLLSQAHSRHSGLSSGSTQERMKAQLNPHLNLLTPARLPSGHAPGQDRIALEPPRTSPNLLLAGEAPSTRPQREAEGEPTARTSTSGSSDLPKAPGGHLHLQARRAGQNSDAGPPRGLTWVPEGAGPVLGGCGRGMDQSCLSADGAGRGCGRATWSVREEQVKQWAAEMLVALEALHEQGVLCRDLHPGNLLLDQAGHIRLTYFGQWSEVEPQCCGEAVDNLYSAPEVGGISELTEACDWWSFGSLLYELLTGMALSQSHPSGIQAHTQLQLPEWLSRPAASLLTELLQFEPTRRLGMGEGGVSKLKSHPFFSTIQWSKLVG

• Full Name: ribosomal protein S6 kinase-like 1
• Calculated Molecular Weight: 549 aa, 60 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC004540
• Gene Symbol: RPS6KL1
• Gene ID (NCBI): 83694
• RRID: AB_2879706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6S9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.