RPS7 Recombinant monoclonal antibody

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  WB analysis using 87092-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87092-1-RR (RPS7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 87092-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS7 antibody (87092-1-RR, Clone: 252258A12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RPS7 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87092-1-RR

Clone No.252258A12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

40S ribosomal protein S7, DBA8, ribosomal protein S7, RPS 7, Small ribosomal subunit protein eS7

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Tested Applications

• Positive WB detected in: HuH-7 cells, HT-29 cells, LoVo cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87092-1-RR targets RPS7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5999

  Product name: Recombinant human RPS7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC061901

  Sequence: MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL

• Full Name: ribosomal protein S7
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC061901
• Gene Symbol: RPS7
• Gene ID (NCBI): 6201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62081
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S7(RPS7) belongs to the RPs, which is structural compoent of the ribosome invilved in protein synthesis. It interacts with and inhibit mdm2 mediated p53 degradation function. Also RPS7 has a essential role in early development. RPS7 involves in the maturation of ribosomal RNAs in the large or the small ribosomal subunit production pathway.