RRAS Antibody

RRAS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11647-1-AP

Synonyms

p23, Ras related protein R Ras, RRAS



Tested Applications

Positive WB detected inhuman spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

11647-1-AP targets RRAS in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAS fusion protein Ag2246
Full Name related RAS viral (r-ras) oncogene homolog
Calculated molecular weight 218aa,23 kDa
Observed molecular weight 25 kDa
GenBank accession number BC016318
Gene symbol RRAS
Gene ID (NCBI) 6237
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRAS antibody 11647-1-APDownload protocol
IHC protocol for RRAS antibody 11647-1-APDownload protocol
IP protocol for RRAS antibody 11647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB

BMC Microbiol

Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway.

Authors - Yanan Li
humanWB

Sci Rep

Mechanisms of Gefitinib-mediated reversal of tamoxifen resistance in MCF-7 breast cancer cells by inducing ERα re-expression.

Authors - Xia Zhang