RREB1 Polyclonal antibody

RREB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27706-1-AP

Synonyms

FINB, HNT, LZ321, RREB 1, RREB1, Zep 1

---

Validation Data Gallery

• 

  WB analysis using 27706-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27706-1-AP (RREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27706-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27706-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27706-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27706-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27706-1-AP (RREB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, LNCaP cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27706-1-AP targets RREB1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RREB1 fusion protein Ag26780
• Full Name: ras responsive element binding protein 1
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_001003698
• Gene Symbol: RREB1
• Gene ID (NCBI): 6239
• RRID: AB_2880948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family.RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. RREB1 may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)