RRP1 Polyclonal antibody

RRP1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 14896-1-AP

Synonyms

D21S2056E, NNP 1, NNP1, NOP52, Novel nuclear protein 1, Nucleolar protein Nop52, RRP1, RRP1 like protein, RRP1A

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Validation Data Gallery

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  WB analysis of HEK-293 using 14896-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 14896-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14896-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14896-1-AP

  Immunofluorescent analysis of HeLa cells using 14896-1-AP (RRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human spleen tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14896-1-AP targets RRP1 in WB, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRP1 fusion protein Ag6686
• Full Name: ribosomal RNA processing 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC000380
• Gene Symbol: RRP1
• Gene ID (NCBI): 8568
• RRID: AB_2180625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.