RRP8 Polyclonal antibody

RRP8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20129-1-AP

Synonyms

Nucleomethylin, NML, KIAA0409, hucep-1, EC:2.1.1.-

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Validation Data Gallery

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  WB analysis of human brain using 20129-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20129-1-AP (RRP8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20129-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20129-1-AP (RRP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 20129-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20129-1-AP (RRP8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 20129-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20129-1-AP (RRP8 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20129-1-AP

  Immunofluorescent analysis of Hela cells, using RRP8 antibody 20129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20129-1-AP targets RRP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRP8 fusion protein Ag13994
• Full Name: ribosomal RNA processing 8, methyltransferase, homolog (yeast)
• Calculated Molecular Weight: 456 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC001071
• Gene Symbol: RRP8
• Gene ID (NCBI): 23378
• RRID: AB_10665422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cancer Res Clin Oncol; RRP8, associated with immune infiltration, is a prospective therapeutic target in hepatocellular carcinoma; Authors - Kai You