RSK4 Polyclonal antibody

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  WB analysis using 21877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21877-1-AP (RSK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-12 using 21877-1-AP

  IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with PC-12 cells lysate 1560 ug.

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  IP experiment of MCF-7 using 21877-1-AP

  IP result of anti-RSK4 (IP:21877-1-AP, 4ug; Detection:21877-1-AP 1:800) with MCF-7 cells lysate 1520 ug.

RSK4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 21877-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

RPS6KA6, p90RSK6, pp90RSK4, S6K alpha 6

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: MCF-7 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21877-1-AP targets RSK4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16299

  Product name: Recombinant human RPS6KA6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 629-745 aa of BC148802

  Sequence: TPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL

• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 6
• Calculated Molecular Weight: 745 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC148802
• Gene Symbol: RPS6KA6
• Gene ID (NCBI): 27330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UK32
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal s6 kinase4 (RSK4), an X-linked gene, firstly was found as to be a potential tumor suppressive gene in a variety of cancers and is widely participated in signaling pathway. RSK4 is a predominantly cytosolic protein with very low expression and several characteristics of the RSK family kinases, including the presence of two functional kinase domains and a C-terminal docking site for ERK (PMID: 39114716, 30579335). Endogenous RSK4 showed the same level of phosphorylation of Ser232, Ser372, and Ser389 in starved and growth factor-stimulated cells, and mutational analysis demonstrated that these sites are important in stimulation of kinase activity (PMID: 15632195).