RSRC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RSRC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23826-1-AP

Synonyms

SRrp53, Serine/Arginine-related protein 53, BM-011, BM 011, Arginine/serine-rich coiled-coil protein 1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 23826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23826-1-AP (RSRC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23826-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23826-1-AP (RSRC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 23826-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 23826-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 23826-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 23826-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23826-1-AP (RSRC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23826-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RSRC1 antibody (23826-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human small intestine tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

23826-1-AP targets RSRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSRC1 fusion protein Ag20604
• Full Name: arginine/serine-rich coiled-coil 1
• Calculated Molecular Weight: 334 aa, 39 kDa
• Observed Molecular Weight: 38 kDa, 50 kDa
• GenBank Accession Number: BC006982
• Gene Symbol: RSRC1
• Gene ID (NCBI): 51319
• RRID: AB_2879329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSRC1, also named as SRRP53, is a novel SR-related protein of an apparent molecular mass of 53 kDa (45-53 kDa). It is isolated in a gene trap screen that identifies proteins which localize to the nuclear speckles. RSRC1 plays a rol in pre-mRNA splicing and constitutive.

Publications

Species	Application	Title
human	WB,IHC	Mol Med Rep; RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression.; Authors - Shijun Yu ; KD Validated