RTEL1 Polyclonal antibody

RTEL1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25337-1-AP

Synonyms

Novel helicase like, NHL, KIAA1088, EC:3.6.4.12, C20orf41

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Validation Data Gallery

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  WB analysis of BGC-823 using 25337-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25337-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25337-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25337-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25337-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25337-1-AP

  IP result of anti-RTEL1 (IP:25337-1-AP, 4ug; Detection:25337-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 25337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTEL1 antibody (25337-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: BGC-823 cells, C6 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, PC-3 cells
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

25337-1-AP targets RTEL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: regulator of telomere elongation helicase 1
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: NM_016434
• Gene Symbol: RTEL1
• Gene ID (NCBI): 51750
• RRID: AB_2880034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
zebrafish	WB	Nucleic Acids Res; Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.; Authors - Jingzhen Li
human	WB	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang ; KD Validated
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio